Monoclonal antibody R5 for detection of putatively coeliac-toxic gliadin peptides
نویسندگان
چکیده
منابع مشابه
Measurement of gluten using a monoclonal antibody to a coeliac toxic peptide of A-gliadin.
BACKGROUND Future European Community regulations will require a sensitive and specific assay for measurement of coeliac toxic gluten proteins in foods marketed as gluten-free. To avoid spurious cross reactions with non-toxic proteins, specific antibodies and target antigens are required. A synthetic 19 amino acid peptide of A gliadin has been shown to cause deterioration in the morphology of sm...
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The diagnostic value in coeliac disease of circulating antibodies to casein, crude gliadin, and alpha gliadin was assessed using an adaption of the enzyme linked immunosorbent assay system. alpha Gliadin was the only antigen which consistently separated 26 patients with untreated coeliac disease from 26 normal controls and 13 patients with chronic inflammatory bowel disease. The mean assay inde...
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objective : the aim of study was to develop a rapid assay, dye labelled monoclonal antibody assay (dlmaa), using non-radioactive organic synthetic dyes for identification of toxic shock syndrome toxin-1 (tsst-1) producing strains of staphylococcus aureus. materials and methods: the assay protocol required only two simple steps; addition of tsst-1 antigen to a nitrocellulose membrane and then ad...
متن کاملEpithelial Transport of Immunogenic and Toxic Gliadin Peptides In Vitro
SCOPE Celiac disease is an autoimmune disorder caused by failure of oral tolerance against gluten in genetically predisposed individuals. The epithelial translocation of gluten-derived gliadin peptides is an important pathogenetic step; the underlying mechanisms, however, are poorly understood. Thus, we investigated the degradation and epithelial translocation of two different gliadin peptides,...
متن کاملSimultaneous Detection of IgA/IgG AntiTissue Transglutaminase/Deamidated Gliadin Peptides in Serodiagnosis of Celiac Disease
Background: Celiac disease is a common autoimmune disorder that is diagnosed based on clinical case identification, serological screening, and duodenal histology. However, the existence of mild clinical forms, such as seronegative cases with patchy atrophy and potential celiac disease, can make it difficult to determine a definitive diagnosis. The seronegative patients with celiac disease can i...
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ژورنال
عنوان ژورنال: European Food Research and Technology
سال: 2006
ISSN: 1438-2377,1438-2385
DOI: 10.1007/s00217-006-0268-2